From syringe to print using ryeby
polish transl. www.psilosophy.info/gveihzgyacbhambjclacbgic
original source: https://www.shroomery.org/8432/From-syringe-to-print-using-rye
Table of Contents:
I know there is a lot of steps in this and it's a lot of overkill but it does leave little room for contaminations.
1. First thing I do is put one cup of rye in each quart jar.
2. Put in enough water for the soaking. Alternatively just measure out the grain and let it soak in a big pot (for instance the same pot you are going to use for the simmering later).
I like to soak for 24 hrs. Notice the difference in the color of the water after 24 hrs.
Bacteria endospores have started to germinate.
This is the reason we soak the grain. Many believe that this is where water is absorbed by the grain but this is only partially true since a substantial amount of water is absorbed by the grain when it is simmered. The main reason for the soaking is that we are germinating the bacterial endospores because they sometimes can survive the pressure cooking process. NOTE: In hot weather (summer) rye will sometimes begin to sprout before the 24 hour soak is over. This is NOT something you want to hapen. In this case shorten the soak time to 12 or 16 hours.
3. Strain the grain through a colander to get rid of the dirty water.
4. Strain the simered grain through a colander. I like to pour 6 gallons through the grain in order to get rid of the starch on the outside of the kernels. (no reason for 6 it's just a me thing). After the rinsing let the grain sit in the collander for ~10 minutes so the excess water can run off. The grain is now ready to be put into jars and pressure cooked.
5. Make 2 holes in the jar lids. One is a breather and one is for the syringe needle.
Put a piece of Tyvek over the top, add the ring and some foil and your ready to PC.
Be sure to leave room in the jars to shake the grain.
6. Load the jars into the PC
and pressure cook for 1 hour at 15 psi. Here is a finished jar.
Now your ready for the next step.
1. First thing get your bottom tub and put in your heater. I also add a water pump to mix the water that way it heats up more evenly but it isn't a necessary item. I got the tape from a boat shop and it has been working for some time. Good stuff for underwater.
2. Next put something (I used tall half pints) in each corner of the tub. This will keep the heater and pump safe from being smashed if I put in to many jars or do something stupid.
3. Now be sure to set the heater before you put on the top tub. I attach the top tub with zip ties cause the top tub will want to float.
4. Add the water. I like to add as much water as I can before it starts to spill out.
5. If you use the heaters with the external controls set them now to get ready for the jars. Mine use a dial and a sensor that gives the temp inside the chamber rather then the water. It also has a metal bulb rather then a glass one.
1. First get your work area ready.
2. One of the reasons I like Tyvek is cause you can write on it
3. I like to heat the needle tip then cool it with a alcohol wipe.
4. Inoculate the grain. I like to use 2.5 cc per quart.
5. I like to cover the inoculation hole with a band-aid. I like em cause they come sterile.
6. Shake up the jars real good and put em in a 82 F - 27.7C to 86F - 30C incubator.
7. I like to shake the jars again when they are about 25% colonized then once more at about 50% to 75% done.
1. This is the hard part, the waiting game. Here is a jar 3 ½ days after being shot up.
Few days later.
The first part of the waiting game is over time to
1. Well it's been exactly 9 days and the jars are done.
Time to get the casing supplies.
2. I like coco coir and vermiculite mix. Be sure you get coir and not fiber. Coir is on the left and fiber is on the right.
Coir in the package.
Vermiculite can be found all over the place and looks like this.
3. Add water to the coir as the directions say. Here is a brick.
Here is one with 4 quarts of water added.
4. Prepare your vermiculite. I just put it in a jar and fill it with water, let it soak,
drain then add to a pot.
5. Now mix (I used 2 parts coir to 1 part vermiculite). Here it is when mixed.
6. Put the mix into jars (I just use the grain jars) and pressure cook for 1 ½ hrs at 15 psi. (Once again just something I do)
Usually this last 4 to 7 days. A finished casing should look a little like this
(I suggest covering the sides of clear containers to prevent side pinning. These will be left uncovered for pictures used in this post)
Time to put the casings in.
1. When the mycelium starts to break the casing surface is when I like to put them in the chamber.
Lots of people like to patch at this time. Myself I've never been one for patching so in the chamber they go.
2. I like to use the tent style chamber that uses the cool mist running all the time and a ultrasonic that comes on every few hours for so many minutes.
3. This is a Martha Stewart closet and hose set up.
I use the ultrasonic and cool mist. Very firm believer in air exchange.
In the electrical Dept of the hardware store I find these.
Now on the back of the closet I cut the holes for the hose fittings and put tape over them and cut holes threw the tape for the fittings. The reason for the tape is to give the closet plastic a little extra strength when holding the hose weight.
The outlet for the ultrasonic goes above the cool mist outlet that way the mist from the ultrasonic drops into the air stream of the cool mist and mixes better.
4. Now that the casings are in the chamber it's time to wait for pinning.
5. If the coir starts to get a little lighter in color it may be starting to get dry. Now is a good time to mist them with some sterile water, it's safe to mist up to the point of pinning.
Well it's been a week and pinning has started.
A few days later.
Well some of the veils are starting to tear
so it's time to think about.
Now I suggest you take your own prints to use.
That way when the print is done you have these flaps to fold over.
I then get a half pint jar (wide mouth) and prepare it like this. I ditch the disk and silicone in a Tyvek disk.
This will allow the cap to dry a little as sits in the jar. (Which causes more spores to drop and prevents the cap from rotting. Will also allow the prints to dry once the mushroom cap is removed.)
Next put in the foil and cover with the lid.
Put some foil on top and pressure cook for one hour at 15 psi.
Cut the shroom just below the cap and put it on the foil in the jar as fast as you can. Easy to do since the jar lid is loose.
Why do you wrap a shot glass for each syringe? This is more of a me thing but I like one glass for each syringe just in case there is a contamination I can keep it somewhat contained to one syringe.
Why do you soak the grain in separate jars? This is another one of those me things. I like to come by every once and a while and shake up the jars. No idea if it helps but it makes me feel better and I get a good look at all the grain being used.
Why do you pour out the water and fill and shake and pour that out after the grain has soaked? I like to be sure there is nothing bad like a endospore that may not have germinated and to rinse the water the grain was soaking in out of the jar.
When the grain is done simmering why do you rinse it with 6 gallons of water? Well 6 gallons was used for every 10 quarts of substrate prepared for this post. 6 gallons isn't needed but I do suggest rinsing off the grain.
Why do you leave room in the jars to shake the grain? The jars are shaken to spread the spores around and when the mycelium has started to grow to spread it out so colonization is done faster.
When you inoculate the grain you heat the needle tip then cool it with a alcohol wipe, is it needed to do both? No, if you like you can use one or the other.
When you prepare the vermiculite for the casing you soak it in a jar is that the only way to do it. No, that is just a easy way for me to do it. Anything that can hold the vermiculite and water will pretty much work. I like the vermiculite to have as much water in it as possible.
Is pressure cooking the casing layer the only way to sterilize it before using? No, that is just what I like to do and haven't had a contamination problem using it. There are several other ways to do it and they can be found here in the FAQ.
In the post when you put the casing together you have it in a clear container. Yes that was just done for the post. Light should be shielded from the sides of the casing.
When the mycelium starts to break the casing surface is when I like to put them in the chamber. What about patching? I'm not one that feels that patching is all that needed but many people do and how to do it is in the FAQ.
With your chamber you use two humidifiers, is this needed? I think so to get the most out of your shrooms. The cool mist exchanges the air while not dropping the humidity to much and every few hours the ultrasonic comes on to make sure the humidity is high enough. If you use only one, use the cool mist humidifier.